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Molecular studies at PhyMedExp

Molecular studies at PhyMedExp

Responsable : Cécile Notarnicola, Magalie Taulan-Cadars

The PhyMedExp laboratory has the expertise and equipment necessary for mutation research and gene expression studies.

  • Dosage and quality control of nucleic acids (Nanodrop, Bioanalyzer)

The validation step of the quantity and quality of the nucleic acids (RNA/DNA) is essential to ensure the quality of the results.

The assay can also be performed in 16-well (NanoQuant Tecan) or 96-well (Multiskan) plates.

  • Real-time PCR (qPCR):

Real-time PCR, also known as quantitative PCR or qPCR, is a simple and accurate method for detecting a target DNA sequence and quantifying the expression of a gene present in a sample (LC480 in 96 and 384 well plates).

  • DNA methylation analysis (PyromarkQ24 Quiagen):

Pyrosequencing is a technique that allows the quantitative analysis of DNA methylation at loci of interest.

We also have access to Sanger and NGS sequencers (Miseq, NextSeq) for mutations/variant search and RNA profiling (lncRNA, miRNA).

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