Expertise
Nanovesicles
Generation, characterization, and evaluation of their usefulness as biomarkers and drug-delivery therapy tools
Biomarker screening
- Characterization of nanovesicles as crucial actors during the development of diseases related to metabolic disorders
- From bed to bench and bench to bedside: Identification of new biomarkers to improve the diagnosis, prognosis, and treatment of the patients
- Using different approaches (ex vivo, in vitro, and in vivo), evaluation of their implication in the molecular alterations associated with pathological conditions
Nanotherapeutical Tool
- Nanovesicles as a therapeutic tool for treating different diseases
- Nanovesicles can be edited to precisely target a specific tissue to improve the delivery of their content to increase the efficiency of the treatment and decrease its undesirable effects related to a systemic administration
- To develop a nanomedicine approach to harness the biology of nanovesicle as an innovative approach to assess suitable tool for specific targeting of the hypothalamic areas modulating energy balance
Our Strategy
Generation
- Nanovesicles are obtained from different types of samples:
- Blood
- Feces
- Biopsy
- Cell culture supernatant (under basal or mimicking pathological conditions)
- Isolation by sequential high-speed centrifugation
Characterization
- Numeration and size distribution analysis
- Validation of nanovesicle-subtypes enrichment evaluating specific markers
- Specific analysis of the molecule of interest (proteins, miRNA, …) carried by nanovesicles
Evaluation
- Analysis of the molecular targets using primary and established cell lines from murine and human origins
- Use different animal models to validate the role of nanovesicles
