We are currently studying potential defects in muscle function (regeneration, differentiation, contraction) using this cell model subjected to various stimulations (Catteau et al. 2021).
Objective 2 : Impact of the inflammatory microenvironment on muscle dysfunction in COPD patients
We will evaluate the level of expression of a panel of pro-inflammatory molecules in the serum of COPD patients of different stages (mild, severe, in exacerbation) in order to determine target biomarkers. The deleterious effect on the muscle of these different microenvironments, depending on the inflammatory profile of the patients, will then be tested on our cellular model by studying different signaling pathways (regeneration, differentiation, atrophy) with the aim of targeting a potential therapeutic candidate (Catteau et al. 2020).
Objective 3 : An animal model of COPD: the elastase-LPS rat
We are currently using an elastase-LPS rat model, common to the team, which mimics the pulmonary and extra-pulmonary alterations of COPD. We are currently evaluating the effects of elastase-LPS treatment on muscle alteration (contractility in vivo and in vitro, atrophy). This animal model will allow us to evaluate the therapeutic effects of various molecules and non-drug interventions on skeletal muscle.
Keywords : COPD, skeletal muscle, atrophy, micro-environment, cell model, satellite cell, inflammation, biomarkers, animal model.